Eppendorf Mastercycler® X50

Mastercycler® X50

Artikelnr: EP-Mastercycler®-X50 Kategori:

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Eppendorf Mastercycler® X50

Available as standalone units, controlled by the user-friendly VisioNize® touchscreen interface, or externally controlled eco units, the Mastercycler X50 family offers the flexibility to meet all your throughput needs.Each standalone PCR thermocycler can be networked with up to nine additional eco units. For even higher throughput, you can control up to fifty MC X50 eco units with our CycleManager X50 software. Whichever you need, you’ll be able to monitor and manage every unit – individually, simultaneously or as a group – with the assurance that every PCR cycler provides excellent block homogeneity, accuracy and precision. For security, simply choose different levels of access for different users. Low noise and power usage, and the versatile flexlid® concept for maximized consumable flexibilty, make the MC X50 the most powerful and convenient PCR thermocycler set-up.

Advanced PCR Optimization in a One Step

Mastercycler X50 PCR thermocyclers have a 2D-gradient function that means you can simultaneously optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) – see the example of β-actin gene PCR optimization.

Higher denaturation temperatures can increase specificity while lower denaturation temperatures can increase yield, so with the MC X50 PCR cyclers, you can quickly determine the conditions for both optimal yield and specificity. This saves PCR optimization time, is a great benefit in low target copy number and GC-rich target applications, and is really useful in troubleshooting non-specific amplification issues.

Mastercycler® X50

Need rapid PCR results?

In the modern lab, fast is essential, not a luxury. PCR thermocyclers that give you quicker results throughout the day and a faster transition to optimal temperatures mean you can get through more work in a shorter time. The Mastercycler X50 PCR cycler heats at 10 °C/s and cools at 5 °C/s, so make the speed of the MC X50 your research advantage.

Mastercycler® X50

Speed is more than just the ramp rate

There is a wide range of PCR thermocyclers available, with differing claims about ramp rates. However, if you compare the same protocol using different PCR cyclers, you see that ramp rates don’t tell the full story about run time speed. At Eppendorf, we don’t just make claims, we back them up; so we’ve done the performance comparison which shows that the Mastercycler X50 really does give you reliable ramp rates and real PCR speed. To see the full report, see Application Note 274.

Mastercycler® X50

Improving ergonomics across the lab

Ergonomics goes far beyond just the »ergonomically designed chair«. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.

In 2003, we launched the PhysioCare Concept®, focusing on ergonomic liquid handling devices, like our pipettes.

Nowadays, the Eppendorf PhysioCare Concept has broadened to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and well-being.

For the Eppendorf Mastercyler range, this means:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling

Mastercycler® X50

Simple, intuitive documentation and tracking

The eLABJournal® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Mastercycler® X50


Eppendorf Mastercycler® X50

MC X50s MC X50a MC X50p MC X50h MC X50i MC X50l MC X50r MC X50t
Touchscreen Interface
Unit can control 9 other units in network
Unit can only be controlled
Thermoblock Silver Aluminum Aluminum Aluminum Silver Aluminum Aluminum Aluminum
Sample capacity 96-well plate or 0.1/0.2 mL tubes 96-well plate or 0.1/0.2 mL tubes 1 PCR plate 96 1 PCR plate 384 96-well plate or 0.1/0.2 mL tubes 96-well plate or 0.1/0.2 mL tubes 1 PCR plate 96 1 PCR plate 384
96-well plate
384-well plate
0.1/0.2 mL tubes
Heating technology Six peltier elements Six peltier elements Six peltier elements Six peltier elements Six peltier elements Six peltier elements Six peltier elements Six peltier elements
2D-Gradient block over 12 columns/over 8 rows over 12 columns/over 8 rows over 12 columns/over 8 rows over 24 columns/over 16 rows over 12 columns/over 8 rows over 12 columns/over 8 rows over 12 columns/over 8 rows over 24 columns/over 16 rows
Temperature control mode Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe Fast, Intermediate, Standard, Safe
Temperature control range of the block 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C
Gradient range 1 – 30 °C 1 – 30 °C 1 – 30 °C 1 – 30 °C 1 – 30 °C 1 – 30 °C 1 – 30 °C 1 – 30 °C
Gradient temperature control range 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C
Block homogeneity 20 – 72 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C ≤±0.2 °C
Block homogeneity 95 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C ≤±0.3 °C
Control accuracy ±0.15 °C ±0.15 °C ±0.15 °C ±0.15 °C ±0.15 °C ±0.15 °C ±0.15 °C ±0.15 °C
Cooling rate 5 °C/s 2.3 °C/s 2.3 °C/s 2.3 °C/s 5 °C/s 2.3 °C/s 2.3 °C/s 2.3 °C/s
Lid descent flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP flexlid® technology with TSP
High pressure lid
Lid temperature range 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C
Interfaces Ethernet, USB Ethernet, USB Ethernet, USB Ethernet, USB Ethernet, USB Ethernet, USB Ethernet, USB Ethernet, USB
Power supply 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz 110 – 230 V, 50 – 60 Hz
Max. power consumption 850 W 850 W 850 W 850 W 850 W 850 W 850 W 850 W
Total weight 11.5 kg 11.5 kg 11.5 kg 11.5 kg 10.7 kg 10.7 kg 10.7 kg 10.7 kg
Dimensions (W × D × H) 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in 27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in
Eppendorf ThermoMixer®
Heating rate 10 °C/s 5 °C/s 5 °C/s 5 °C/s 10 °C/s 5 °C/s 5 °C/s 5 °C/s
Brochure – Master of Class
Brochure – Simple, Central, Smart
Brochure – The Next Stage
Customer Testimonial – Interview Henner Tasch
Eppendorf produktkatalog

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