Beskrivning

Eppendorf – CCCadvanced® FN1 motifs Cell Culture Plates

Cultivating iPSCs, MSCs, primary cells or other ECM-dependent cells? Tired of lot-to-lot coating media variation/validation, tedious preparation and increased contamination risk? How about a defined synthetic, xeno-free surface for stable long-term expansion of your stem cells?CCCadvanced^® FN1 motifs cell culture plates are ready-to-use. They can be also used under restrictive culture conditions (serum- and xeno-free media).

CCCadvanced FN1 motifs consumables are classical Eppendorf Cell Culture Consumables, additionally coated with a defined synthetic animal- and human-component-free substrate. The substrate (fibronectin-derived motifs with optimized steric configuration) is designed to mimic the cell attachment site of native ECM.Learn more about cultivation of human iPSCs and MSCs on FN1 motifs and smart features of our cultureware below.

iPSCs: Efficient long-term expansion in a completely synthetic culture system

› Supports efficient long-term hiPSC expansion in a completely defined, animal-, and human-component-free culture system for 25 passages
› Consistent and robust growth rate
› Typical morphology remains stable (Fig. 1)
› hiPSCs remain undifferentiated and maintain functional pluripotency
› Maintenance of trilineage differentiation potential after long-term expansion while exhibiting normal genomic integrity
› For further details see Application Note 389 under “Notes & Papers” below

MSCs: Animal-component-free expansion of human mesenchymal stem cells

› Supports efficient hMSC proliferation in a completely animal-component-free environment even on long term (up to 10 passages)
› Characteristic morphology (Fig. 2) remains stable for 10 successive passages without signs of replicative senescence
› Stable and robust proliferation rate
› Validated with hMSC from different tissue origins
› Undifferentiated hMSCs retain their multi-lineage differentiation potential after expansion
› For further details see Application Note 390 under “Notes & Papers” below

Optimized microscopical performance

The enhancement of the planarity, superior material clarity and reduction of the meniscus of liquids in the wells facilitate every step where manual or automated read-out is necessary. Shadow interference in imaging area is significantly reduced as shown in comparison to a competitor plate.

Safe handling

A pronounced corrugation of the plate lid and a smaller lid makes working with Eppendorf Cell Culture Plates extremely comfortable and safe.

Eppendorf – CCCadvanced® FN1 motifs Cell Culture Plates

FN1 motifs 6-well plate	FN1 motifs 24-well plate	FN1 motifs 96-well plate
Material	polystyrene, meets requirements of USP Class VI	polystyrene, meets requirements of USP Class VI	polystyrene, meets requirements of USP Class VI
Number of wells	6 wells	24 wells	96 wells
Operating temperature	15 °C – 37 °C	15 °C – 37 °C	15 °C – 37 °C
Packing	5 plates, individually wrapped	5 plates, individually wrapped	5 plates, individually wrapped
Purity grade(s)	Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic	Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic	Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic
Surface	Coated with synthetic fibronectin-derived motifs	Coated with synthetic fibronectin-derived motifs	Coated with synthetic fibronectin-derived motifs
Xeno-free	Manufactured using animal- and human-component-free materials	Manufactured using animal- and human-component-free materials	Manufactured using animal- and human-component-free materials
Dimensions (W × D × H)	127.8 mm × 85.5 mm ×	127.8 mm × 85.5 mm ×	127.8 mm × 85.5 mm ×
Storage temperature	Store dry at room temperature from 15 °C to 30 °C	Store dry at room temperature from 15 °C to 30 °C	Store dry at room temperature from 15 °C to 30 °C
Theoretical total volume	16 mL	3.6 mL	0.4 mL
Working volume	3.0 – 5.0 mL	0.5 – 1.0 mL	0.1 – 0.2 mL
Growth area/well¹	940.3 mm²	208.9 mm²	37.0 mm²
Well diameter	34.6 mm	16.2 mm	6.8 mm
Height with lid	23.2 mm	23.2 mm	17.6 mm
Dimensions	Conforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions	Conforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions	Conforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions
Centrifugation	Centrifugable up to 2,500 × g Centrifugable in stack (4 plates) up to 300 × g	Centrifugable up to 2,500 × g Centrifugable in stack (4 plates) up to 300 × g	Centrifugable up to 2,500 × g Centrifugable in stack (4 plates) up to 300 × g

¹For the initial seeding of cells, take into account the growth area in order to support optimal cell growth.

Brochure – Improve Now

Brochure – Seed. Proceed. Reproduce.

Flexible and Xeno-Free hiPSC Culture System

Eppendorf produktkatalog

LEASING / HYRA / AVBETALNING

Vårt samarbete med Svea gör att du snabbt och enkelt får förslag på finansiering som passar dina behov Finansiering upp till 100%. Ni slipper binda ert kapital. Läs mer om leasing och allriskförsäkring.

Investera nu i rätt utrustning, för att säkra er konkurrenskraft, kapacitet och tillgång till vital utrustning Sprid kostnaden över den tid som utrustningen används. Utrustningen som säkerhet för finansieringen.

Kontakta oss redan idag för ett förslag!