Name: Eppendorf - CCCadvanced® FN1 motifs Cell Culture Flasks - LabTeamet
SKU: EP-CCCadvanced®-FN1-motifs-Cell-Culture-Flasks

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Eppendorf – CCCadvanced® FN1 motifs Cell Culture Flasks

Cultivating iPSCs, MSCs, primary cells or other ECM-dependent cells? Tired of lot-to-lot coating media variation/validation, tedious preparation and increased contamination risk? How about a defined synthetic, xeno-free surface for stable long-term expansion of your stem cells?CCCadvanced^® FN1 motifs cell culture flasks are ready-to-use. They can be also used under restrictive culture conditions (serum- and xeno-free media).

CCCadvanced FN1 motifs consumables are classical Eppendorf Cell Culture Consumables, additionally coated with a defined synthetic animal- and human-component-free substrate. The substrate (fibronectin-derived motifs with optimized steric configuration) is designed to mimic the cell attachment site of native ECM.Learn more about cultivation of human iPSCs and MSCs on FN1 motifs and smart features of our cultureware below.

iPSCs: Efficient long-term expansion in a completely synthetic culture system

› Supports efficient long-term hiPSC expansion in a completely defined, animal-, and human-component-free culture system for 25 passages
› Consistent and robust growth rate
› Typical morphology remains stable (Fig. 1)
› hiPSCs remain undifferentiated and maintain functional pluripotency
› Maintenance of trilineage differentiation potential after long-term expansion while exhibiting normal genomic integrity
› For further details see Application Note 389 under “Notes & Papers” below

MSCs: Animal-component-free expansion of human mesenchymal stem cells

› Supports efficient hMSC proliferation in a completely animal-component-free environment even on long term (up to 10 passages)
› Characteristic morphology (Fig. 2) remains stable for 10 successive passages without signs of replicative senescence
› Stable and robust proliferation rate
› Validated with hMSC from different tissue origins
› Undifferentiated hMSCs retain their multi-lineage differentiation potential after expansion
› For further details see Application Note 390 under “Notes & Papers” below

Cell accessibility

A unique ConvexAccess™ neck geometry significantly facilitates the access to the growth area and makes cell seeding and media exchange more convenient, safe and reliable

Improved contamination protection

The new hydrophobic volume filter pad is more efficient than standard filter membranes

Corrugated cap

Anti–rolling cap with corrugation and clear position marks in plug–seal caps to prevent undesired full–closing

Eppendorf – CCCadvanced® FN1 motifs Cell Culture Flasks

FN1 motifs T-75 flask	FN1 motifs T-175 flask
Material	polystyrene, meets requirements of USP Class VI	polystyrene, meets requirements of USP Class VI
Operating temperature	15 °C – 37 °C	15 °C – 37 °C
Packing	5 flasks, individually wrapped	5 flasks, individually wrapped
Purity grade(s)	Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic	Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic
Surface	Coated with synthetic fibronectin-derived motifs	Coated with synthetic fibronectin-derived motifs
Xeno-free	Manufactured using animal- and human-component-free materials	Manufactured using animal- and human-component-free materials
Storage temperature	Store dry at room temperature from 15 °C to 30 °C	Store dry at room temperature from 15 °C to 30 °C
Theoretical total volume	279.8 mL	662.1 mL
Working volume	8.0 – 20.0 mL	20.0 – 30.0 mL
Growth area/well¹	77.9 mm²	179.5 mm²
Dimensions	Conforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions	Conforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions
Centrifugation	Can be centrifuged with the suitable adapters; refer to the manufacturer’s instructions	Can be centrifuged with the suitable adapters; refer to the manufacturer’s instructions

Brochure – Improve Now

Brochure – Seed. Proceed. Reproduce.

Flexible and Xeno-Free hiPSC Culture System

Eppendorf produktkatalog

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